The first Greek civilization, known as Mycenaean (c. 1600-1200 BC) owed much to the Minoan civilization of Crete and may have been produced by the intermarriage of Greek-speaking invaders with the original inhabitants. From the 14th century BC a new wave of invasions began. The Achaeans overran Greece and Crete, destroying the Minoan and Mycenaean civilizations and penetrating Asia Minor; to this period belongs the siege of Troy (c. 1180). The latest of the invaders were the Dorians (c. 1100) who settled in the Peloponnese and founded Sparta; that great city-state arose during the obscure period that followed (1100-800). The mountainous geography of Greece hindered the cities from attaining any national unity, and led the Greeks to take to the sea. During the years 750-550 the Greeks not only became great traders, but founded colonies around the coasts of the Mediterranean and the Black Sea, in Asia Minor, Sicily, S Italy, S France, Spain, and N Africa. Many Greek cities passed from monarchy to the rule of a landowning or merchant oligarchy and thence to democracy. Thus Athens passed through the democratic reforms of Solon (594), the enlightened `tyranny' of Pisistratus (560-527), and the establishment of democracy by Cleisthenes (c. 507). Sparta remained unique, a state in which a ruling race, organized on military lines, tyrannized the original population. After 545 BC the Ionian cities fell under the dominion of the Persian Empire. Aid given them by Athens in an unsuccessful revolt in 499-494 provoked Darius of Persia to invade Greece in 490 only to be defeated by the Athenians at Marathon and forced, to withdraw. Another invasion by the Persian Emperor Xerxes, after being delayed by the heroic defence of Thermopylae by 300 Spartans, was defeated at sea off Salamis in 480 and on land at Plataea in 479. The Ionian cities were liberated and formed a naval alliance with Athens, the Confederacy of Delos. Pericles, the real ruler of Athens 461-429, attempted to convert this into an Athenian empire and to form a land empire in Greece. Mistrust of his ambitions led to the Peloponnesian War (431-404), which destroyed the political power of Athens. In 5th-century Athens, Greek tragedy, comedy, sculpture, and architecture were at their peak, and Socrates and Plato founded moral philosophy. After the Peloponnesian War, Sparta became the leading Greek power until it was overthrown by Thebes (378-371). The constant wars between the cities gave Philip II of Macedon (358-336) the opportunity to establish his supremacy over Greece. His son Alexander the Great overthrew the decadent Persian Empire, conquered Syria and Egypt, and invaded the Punjab. After his death in 323 his empire was divided among his generals, but his conquest had nevertheless spread Greek culture to the Near East. During the 3rd century BC the cities attempted to maintain their independence against Macedon, Egypt, and Rome by forming federations; for example, the Achaean and Aetolian Leagues. Roman intervention began in 212 and ended in the annexation of Greece in 146. Under Roman rule Greece remained a cultural centre, until the emperor Justinian closed the university of Athens in AD 529.